Amino Acid Assays
Group |
Analyts, Analytical Targets |
Analytical Method |
|
Amino acids in food (protein
bond and free amino acids, total) |
Alanine
(Ala), Arginine (ArG), Aspertic
Acid (Asp), Cystine (Cys-Cys),
Glutamic Acid (Glu),
Glycine (Gly), Histidine
(His), Isoleucine (Ile),
Leucine (Leu), Lysine (Lys),
Methionine (Met), Phenylalanine (Phe),
Proline (Pro), Serine (Ser), Threonine (Thr),
Tryptophan (Try), Tyrosine (Tyr), Valine
(Val) |
AOAC 994.12 |
|
Amino acids in supplement
(free amino acids) |
Alanine (Ala), Arginine (ArG),
Aspertic Acid (Asp), Cystine
(Cys-Cys), Glutamic
Acid (Glu), Glycine (Gly),
Histidine (His), Isoleucine (Ile), Leucine (Leu), Lysine (Lys), Methionine (Met), Phenylalanine (Phe), Proline (Pro), Serine (Ser), Threonine (Thr), Tryptophan (Try), Tyrosine (Tyr),
Valine (Val) |
Pre-column OPA derivatization HPLC |
|
Free amino acids in food
or supplement |
Glycine, Alanine, Valine, Leucine, Isoleucine,
Serine, Threonine, Aspartate, Glutamate,
Asparagine, Histidine, Arginine,
Lysine, Ormithine, Phenylalanine, Tyrosine,
Tryptophan, Cysteine (total), Homocysteine (total), Taurine,
Methionine |
HPLC |
Other |
Acetyl-L-Carnitine |
1H-NMR, 300 MHz |
Acetyl Cysteine, N-Acetyl Cysteine |
HPLC-RI |
Alanine |
Perchloric acid titration |
Arginine |
Perchloric acid titration
|
Arginine-alpha-Ketoglutarate, L-Arginine-alpha-Keto-Glutarate |
1H-NMR, 300 MHz |
Arginine Hydrochloride |
Spectrophotometric |
|
Aspartame, L-Aspartyl-L-phenylalanine
methyl ester |
Titration |
|
Aspartame, in formulation |
HPLC |
|
Aspartic Acid |
Titration;
HPLC |
|
Betaine Hydrochloride |
Titration |
|
Choline Bitartrate |
HPLC |
|
Choline Chloride |
HPLC |
|
Creatine |
HPLC |
|
Lysine Acetate |
Perchloric acid titration |
|
Lysine Hydrochloride |
Perchloric acid titration |
|
Methionine |
Perchloric acid titration |
|
Phenylalanine |
Perchloric acid titration |
|
Selenomethionine |
HPLC-UV |
|
Serine |
Perchloric acid titration |
|
Serotonine (5-HT), 5-Hydroxytryptamine |
HPLC |
|
Thiamine Hydrochloride |
HPLC |
|
Thiamine Mononitrate |
HPLC |
|
Threonine |
Perchloric acid titration |
|
Trypsin Inhibitor Activity |
TIU Assay |
|
Tryptophan, L-Tryptophan |
Perchloric acid titration |
|
Tyrosine |
HPLC |
|
Tyrosine |
Perchloric acid titration |
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