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Reagents for mounting
a slide Chloral hydrate - Glycerin. Chloral hydrate 50 g with 10 mL of
glycerin and 15 mL of water. It "re-hydrolyze" tissues and dissolves starch, protein, gum,
essential oils, and chlorophyll. It's one of the most powerful clearing
reagents for microscopic analysis. Ethanol. Use 95% ethanol to examine inulin (alant starch), mucilages, and gums.
Higher ethanol is useful to dissolve oils, resins, and chlorophylls, thus,
acts as a clearing reagent. Glycerin - Acetic acid. Glycerin, acetic acid, and water in 1:1:1 (w/w/w) ratio. It's
useful for observing all microscopic characteristics include starch. Water. Distilled
water is useful for examining many microscopic characteristics. Heat a
powder sample in water can clear out starch. Reagents for examining composition of
cell walls and ergastic contents Alkanna tincture. It's an alcoholic
solution of alkannin (extracted from the root
of Alkanna tinctoria)
also known as dyer's alkanet. It stains oils, fats, and cuticularized
cell walls. Iodine water. Dissolve about
25 mg of iodine in 100 mL distilled water. The
solution dyes starch and hemicelluloses to blue. Phloroglucinol - Hydrochloric acid solution. Freshly prepared 0.1 g phloroglucinol with
1 mL ethanol and 9 mL
hydrochloric acid. The solution is highly corrosive. Use with great
care. It stains lignified cell walls to red. Ruthenium red solution. Freshly prepared
by adding ruthenium red to 2 mL of 10% sodium
acetate aqueous solution until a scarlet color is obtained. It stains
gums and micilages. Zinc chloride - Iodine solution. Dissolve 20
g zinc chloride in 10 mL of water. After adding
and dissolving 2 g potassium iodide in the water solution, add iodine
until saturate. It dyes true cellulose walls to blue and cutinized walls
to yellow. |