Testing Method, Recommended

Food Chemical Analysis

(for both Health Canada & FDA USDA Compliances)

Calories, Total

Calculated based on proximate.

Health Canada & FDA Total Calories = 9xFat (g) + 4xCarbohydrate (g) + 4xProtein (g)

USDA Total Calories = 9xFat (g) + 4x[Carbohydrate (g) – Insoluble dietary fiber (g)] + 4xProtein (g)

Fat, Total

AOAC 996.06, AACC GLC, AOCS GLC or equivalent: total fatty acids as triglycosides

         Saturated Fatty Acids

         trans Fatty Acids

AOAC 996.06, AACC GLC, AOCS GLC or equivalent

Cholesterol

AOAC 994.10, AACC GLC or equivalent

Carbohydrate, Total Carbohydrates

Calculated based on proximate: Total carbohydrates = (Serving size - ash - moisture - protein - fat)

Fibre, Total Dietary Fiber

AOAC 992.16 or 985.29, AACC or equivalent

Sugars

AOAC 980.13 or equivalent

Protein

AOAC 981.10 or 993.13

Vitamin A

AOAC 992.04, AACC or equivalent

Vitamin C

AACC 86-10, or validated Fluorometric or HPLC, or equivalent

Sodium, Calcium, Iron

Validated ICP, AA, or equivalent

Food Chemical Analysis

(Additional Information for Food Nutrients)

Servings per Container

Claimed by the manufacturer

Kilojoules, kJ per serving

Calculated based on proximate (see Calories). 1 Calorie = 4.186 kJ

Calories from Total Fat

Calories from Saturates + trans

Calculated based on a factor of 4 x g of the fatty acid, that’s determined by AOAC 996.06, AACC GLC, AOCS GLC or equivalent

Polyunsaturated Fatty Acids

Omega-6 Polyunsaturates

Omega-3 Polyunsaturates

Monounsaturated Fatty Acids

AOAC 996.06, AACC GLC, AOCS GLC or validated GC methods

Soluble Fiber        

Insoluble Fiber

AACC or  equivalent

Sugar Alcohols, Polyols

Validated HPLC-RI or equivalent

Starch

AACC 76-13, AOAC or equivalent

Vitamin D (Vit D)

Validated HPLC-UV,  or equivalent

Vitamin E (Vit E)

AACC 86-47 or equivalent

Vitamin K (Vit K)

Validated HPLC-UV, or equivalent

Thiamine (Thiamin, Vit B1)

AACC 86-80, or equivalent

Riboflavin (Vit B2)

AACC 86-70/72, or equivalent

Niacin

AACC 86-49/50A/51, or equivalent

Vitamin B6 (Vit B6)

AACC 86-31, or equivalent

Folate

AACC 86-47 or equivalent

Vitamin B12 (Vit B12)

AACC 86-40 or equivalent

Biotin

Validated HPLC-UV or immunoassays

Pantothenic Acid

Validated HPLC-UV or equivalent

Phosphorus, Magnesium, Zinc, Selenium, Copper, Manganese,

Chromium, Molybdenum

Validated ICP or equivalent

Iodide (Iodine)

USP titration or equivalent

Chloride

Validated IC or equivalent